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MABD131 Anti-ISL1 Antibody, clone 1H9

Detect ISL1 using this mouse monoclonal antibody, Anti-ISL1 Antibody, clone 1H9 validated for use in western blotting, Immunofluorescence & IHC.

Anti-ISL1 Antibody, clone 1H9 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Stem Cell Research Research Sub-Category: Pluripotent & Early Differentiation Gene Symbol: ISL1 UniProt Number: P61371
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MABD131
100 µL  
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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IF, IHCMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABD131
      DescriptionAnti-ISL1 Antibody, clone 1H9
      Alternate Names
      • Insulin gene enhancer protein ISL-1
      • Islet-1
      Background InformationIslet-1 (ISL-1) is a transcription factor that plays an important role in the embryogenesis of pancreatic islets of Langerhans. It is expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain. In mouse embryos, a deficiency of this gene fail to undergo neural tube motor neuron differentiation. More recently it has been identified as a marker for a cardiac progenitor cell lineage that is capable of differentiating into all 3 major heart cell types: cardiomyocytes, smooth muscle and endothelial cell lineages.
      References
      Product Information
      FormatAscites
      Control
      • Full-length ISL1-hIgGFc transfected HEK293 cell lysate
      PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect ISL1 using this mouse monoclonal antibody, Anti-ISL1 Antibody, clone 1H9 validated for use in western blotting, Immunofluorescence & IHC.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • Immunohistochemistry
      Application NotesImmunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected ISL1 in full-length ISL1-hIgGFc transfected HEK293 cells.

      Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected ISL1 in paraffin-embedded human lung cancer and cervical carcinoma tissues.

      Optimal working dilutions must be determined by end user.

      Biological Information
      ImmunogenPurified recombinant fragment of human ISL1 expressed in E. Coli. corresponding to human ISL1.
      Clone1H9
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • ISL1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~93 kDa observed. Uncharacterized bands may appear in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in full-length ISL1-hIgGFc transfected HEK293 cell lysate.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ISL1 in full-length ISL1-hIgGFc transfected HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABD131 04053252916458

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      Catégories

      Life Science Research > Antibodies and Assays > Primary Antibodies