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MABT820 Anti-Integrin alpha 5 (CD49e) Antibody, clone mAb16

Anti-Integrin alpha5 (CD49e), clone mAb16, Cat. No. MABT820, is a rat monoclonal antibody that detects Integrin alpha5 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Inhibition assays.

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Research Category: Cell Structure Gene Symbol: ITGAS, FNRA UniProt Number: P08648
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MABT820
100 µL  
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      Replacement Information

      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IP, InhibitionRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT820
      DescriptionAnti-Integrin alpha 5 (CD49e) Antibody, clone mAb16
      Alternate Names
      • CD49 antigen-like family member E
      • Fibronectin receptor subunit alpha
      • Integrin alpha-F
      • VLA-5
      • CD49e
      Background InformationIntegrin alpha-5 (UniProt: P08648; also known as CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49e) is encoded by the ITGA5 (also known as FNRA) gene (Gene ID: 3678) in human. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. Integrin alpha-5 is a single-pass type I membrane protein is expressed on multiple cell types. It contains four calcium binding sites (aa 480-488; 334-342; 401-409; and 465-473). Structurally, it consists of an extracellular domain (aa 42-995), a transmembrane domain (aa 996-1021), and a cytoplasmic domain (aa 1022-1049). When associated with beta 1 subunit it can serve as a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. Integrin alpha-5 is also expressed in articular cartilage and plays a role in synovial joint development and in the regulation of chondrocyte survival and matrix degradation in articular cartilage. Membrane fractions obtained from fibroblasts of diabetic animals display significant reduction in integrin alpha-5 levels. (Ref.: Almeida, ME et al., (2016). Mol. Cell. Biochem. 421(1-2); 19-28).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in PBS without azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Integrin alpha5 (CD49e), clone mAb16, Cat. No. MABT820, is a rat monoclonal antibody that detects Integrin alpha5 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Inhibition assays.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Inhibition
      Application NotesInhibition Analysis: 10 ug/mL from a representative lot prevented HT-1080 cells from adhering to fibronectin coated plates (Courtesy of Dr. Kenneth M. Yamada, M.D., Ph.D., NIDCR, National Institutes of Health, Bethesda, MD USA).

      Inhibition Analysis: A representative lot inhibited WI38 fibroblast attachment to fibronectin (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75).

      Inhibition Analysis: A representative lot inhibited A375-SM cell spreading. (Humphries, J.D., et. al. (2005). J Biol Chem. 280(11):10234-43).

      Immunocytochemistry Analysis: A representative lot detected Integrin alpha5 (CD49e) in Immunocytochemistry applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75).


      Immunoprecipitation Analysis: A representative lot immunoprecipitated Integrin alpha5 (CD49e) in Immunoprecipitation applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75).
      Biological Information
      ImmunogenFibronectin receptor purified from human placenta.
      ClonemAb16
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone mAb16 is a rat monoclonal antibody that specifically detects Integrin alpha5 in human cells.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ITGAS
      • FNRA
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight114.54 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HFF cells.

      Immunocytochemistry Analysis: A 1:25 dilution of this antibody detected Integrin alpha5 (CD49e) in human Foreskin Fibroblast (HFF) cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABT820 04054839416583