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AB1413-I-AF555 Anti-Interleukin-1β Antibody, Alexa Fluor® 555 Conjugate

This rabbit polyclonal Anti-Interleukin-1 Antibody, Alexa Fluor® 555 Conjugate, Cat. No. AB1413-I-AF555, is validated for use in Immunocytochemistry for the detection of Interleukin-1 .

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Research Category: Inflammation & Immunology Gene Symbol: Il1b UniProt Number: P10749
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AB1413-I-AF555
100 µL  
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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MICCRbAlexaFluor®555Polyclonal Antibody
      Description
      Catalogue NumberAB1413-I-AF555
      DescriptionAnti-Interleukin-1β Antibody, Alexa Fluor® 555 Conjugate
      Alternate Names
      • Interleukin-1 beta
      • IL-1 beta
      • IL-1b
      • IL-1beta
      Background InformationInterleukin-1 beta (UniProt; also known as IL-1 beta, IL-1b, IL-1beta) is encoded by the Il1b gene (Gene ID 16176) in murine species. Interleukin-1 (IL-1) is a cytokine that plays a central role in the regulation of acute and chronic inflammation. Two forms of IL-1, IL-1 and IL-1 , are transcribed from two separate genes and mediate similar, but not completely overlapping, biological effects via the membrane-bound IL-1 type 1 receptor (IL-1R1). Studies utilizing Il1a- and Il1b-knockout mice showed that IL-1 , but not IL-1 , is involved in T-cell-dependent antibody production via antigen-specific T-cell activation. IL-1 binding to the extracellular domain of IL1R1 promotes the recruitment of the IL-1 receptor accessory protein and subsequent signal transduction mediated by the cytoplasmic domains of IL-1R1 and IL1-R2. The IL-1 type 2 receptor (IL1R2) is unable to initiate signaling and only acts as a decoy receptor. Soluble receptors for IL-1 (sIL-1Rs) are produced by proteolytic cleavage of membrane-bound receptors or by alternative splicing and act as natural inhibitors by competing against membrane-bound IL1Rs for IL-1 binding. Murine IL-1 beta is produced with a propeptide sequence (a.a. 1-117), which is removed posttranslationally to yield the mature (a.a. 118-269) protein.
      References
      Product Information
      FormatAlexaFluor®555
      PresentationPurified rabbit polyclonal antibody conjugate in buffer containing PBS with 1.5% BSA and 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis rabbit polyclonal Anti-Interleukin-1 Antibody, Alexa Fluor® 555 Conjugate, Cat. No. AB1413-I-AF555, is validated for use in Immunocytochemistry for the detection of Interleukin-1 .
      Key Applications
      • Immunocytochemistry
      Application NotesThe unconjugated antibody (Cat. No. AB1413-I) and Alexa Fluor® 488 conjugate (Cat. No. AB1413-I-AF488) are also available for Immunocytochemistry, Immunoprecipitation, and Western Blotting applications.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a sequence within the C-terminal half of murine and rat mature IL-1beta.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThe unconjugated antibody (Cat. No. AB1413-I) detected both the ~17 kDa mature and ~30 kDa pro-form of murine IL-1beta. The corresponding sequence in human IL-1beta differs from that of the immunogen by only one amino acid. Immunogen sequence is not found in IL-1alpha.
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Gerbil, Hamster, and Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Il1b
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~17/30 kDa observed. 17.39/30.93 kDa (mouse mature/pro-form), 17.32/30.64 kDa (rat mature/pro-form) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in NIH/3T3 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected IL-1beta in 4% paraformaldehyde-fixed, Triton X-100-permeabilized NIH/3T3 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      AB1413-I-AF555 04054839090295