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MABF2767-25UG Anti-MERS-CoV NP Antibody, clone 20

Anti-MERS-CoV NP, clone 20, Cat. No. MABF2767, is a mouse monoclonal antibody that detects nucleoprotein in Middle East respiratory syndrome-related coronavirus (MERS-CoV) and is tested for use in ELISA, Immunoprecipitation, and Western Blotting.

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MABF2767-25UG
25 μg  
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      Replacement Information
      Description
      Catalogue NumberMABF2767-25UG
      DescriptionAnti-MERS-CoV NP Antibody, clone 20
      Alternate Names
      • Nucleoprotein
      • Nucleocapsid protein
      • NC
      • Protein N
      Background InformationNucleoprotein (UniProt: R9UM87; also known as Nucleocapsid protein, NC, Protein N) is encoded by the N gene (Gene ID: 14254601) in Middle East respiratory syndrome-related coronavirus (MERS-CoV). The MERS-CoV is a positive-strand RNA virus of the betacoronavirus genus within the Coronaviridae family that causes severe respiratory syndrome in human. The mature MERS-CoV contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). Nucleoprotein of MERS-CoV is a homooligomeric multifunctional protein that plays roles in genome encapsidation, RNA synthesis during replication, host translational shutoff, and innate immunity disruption. It packages the positive strand viral genome RNA into helical ribonucleocapsid and is involved in virion assembly through its interactions with the viral genome and membrane protein M. The nucleoprotein is reportedly organized into two folded domains known as the N-terminal domain (NTD) and the C-terminal domain (CTD) that are separated by a disordered region (LKR) containing a serine/ arginine stretch. Clone 46 displays specificity for MERS-CoV nucleoprotein and does not show cross-reactivity with nucleoprotein from other related viruses, including SARS coronavirus. (Ref.: Nguyen, THV., et al. (2019). Acta Crystallogr. D. Struct. Biol. 75(1); 8-15; Yamaoka, Y., et al. (2016). Front. Microbiol. 7; Article 509).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-MERS-CoV NP, clone 20, Cat. No. MABF2767, is a mouse monoclonal antibody that detects nucleoprotein in Middle East respiratory syndrome-related coronavirus (MERS-CoV) and is tested for use in ELISA, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • ELISA
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected MERS-CoV NP in Western Blotting applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated MERS-CoV NP in Immunoprecipitation applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).

      ELISA Analysis: A representative lot detected MERS-CoV NP in ELISA applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 292 amino acids (122-413) from the nucleoprotein of Middle East respiratory syndrome-related coronavirus (MERS-CoV).
      EpitopeC-terminal
      Clone#20
      Concentration1.0 mg/mL Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 20 is a mouse monoclonal antibody that detects nucleoprotein of Middle East respiratory syndrome-related coronavirus (MERS-CoV). It target an epitope within 6 amino acids from the C-terminal region.
      IsotypeIgG2b
      Species Reactivity
      • Virus
      Species Reactivity NoteMiddle East respiratory syndrome-related coronavirus (MERS-CoV)
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • N
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~45 kDa observed; 45.05 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant MERS-CoV nucleoprotein

      Western Blotting Analysis: A 1:1,000,000 dilution of this antibody detected recombinant Middle East respiratory syndrome-related coronavirus (MERS-CoV) Nucleoprotein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C . Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABF2767-25UG 04065266298390