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PC542 Anti-MMP-13 (Ab-8) Rabbit pAb

Anti-MMP-13 (Ab-8) Rabbit pAb : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-Collagenase-3, Anti-Matrix Metalloproteinase 13

PC542
  
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Aperçu

Replacement Information

Tableau de caractéristiques principal

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes MMP-13 in breast carcinoma tissue.
Catalogue NumberPC542
Brand Family Calbiochem®
SynonymsAnti-Collagenase-3, Anti-Matrix Metalloproteinase 13
Application Data
Detection of human MMP-13 by staining paraffin sections. Sample: Human breast carcinoma tissue. Primary antibody: Anti-MMP-13 (Ab-8) Rabbit pAb (Cat. No. PC542) (2.5 µg/ml). Detection: DAB, methyl green counterstain.
References
ReferencesBostrom, P.J., et al. 2000. Int. J. Cancer 88, 417.
Hofmann, U.B., et al. 2000. J. Invest. Dermatol. 115, 337.
Mengshol, J.A., et al. 2000. Arthritis Rheum. 43, 801.
Pendas, A.M., et al. 2000. Clin. Chim. Acta 291, 137.
Selvamurugan, N. and Partridge, N.C. 2000. Mol. Cell. Biol. Res. Commun. 3, 218.
Tuckermann, J.P., et al. 2000. J. Bone Miner. Res. 15, 1257.
Winchester, S.K., et al. 2000. J. Biol. Chem. 275, 23310.
Ye, H.Q., et al. 2000. Invest. Ophthalmol. Vis. Sci. 41, 2894.
Barmina, O.Y., et al. 1999. J. Biol. Chem. 274, 30087.
Botos, I., et al. 1999. J. Mol. Biol. 292, 837.
Winchester, S.K., et al. 1999. J. Cell Physiol. 181, 479.
Johansson, N., et al. 1997. Am. J. Pathol. 151, 499.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
Positive controlRecombinant MMP-13 or breast carcinoma tissue
PreservativeNone
Applications
Key Applications Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesImmunoblotting (2.5 µg/ml)
Paraffin Sections (2.5 µg/ml, no pre-treatment required)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human MMP-13
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
PC542 0

Documentation

Anti-MMP-13 (Ab-8) Rabbit pAb Certificats d'analyse

TitreNuméro de lot
PC542

Références bibliographiques

Aperçu de la référence bibliographique
Bostrom, P.J., et al. 2000. Int. J. Cancer 88, 417.
Hofmann, U.B., et al. 2000. J. Invest. Dermatol. 115, 337.
Mengshol, J.A., et al. 2000. Arthritis Rheum. 43, 801.
Pendas, A.M., et al. 2000. Clin. Chim. Acta 291, 137.
Selvamurugan, N. and Partridge, N.C. 2000. Mol. Cell. Biol. Res. Commun. 3, 218.
Tuckermann, J.P., et al. 2000. J. Bone Miner. Res. 15, 1257.
Winchester, S.K., et al. 2000. J. Biol. Chem. 275, 23310.
Ye, H.Q., et al. 2000. Invest. Ophthalmol. Vis. Sci. 41, 2894.
Barmina, O.Y., et al. 1999. J. Biol. Chem. 274, 30087.
Botos, I., et al. 1999. J. Mol. Biol. 292, 837.
Winchester, S.K., et al. 1999. J. Cell Physiol. 181, 479.
Johansson, N., et al. 1997. Am. J. Pathol. 151, 499.
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision11-October-2007 RFH
SynonymsAnti-Collagenase-3, Anti-Matrix Metalloproteinase 13
ApplicationImmunoblotting (2.5 µg/ml)
Paraffin Sections (2.5 µg/ml, no pre-treatment required)
Application Data
Detection of human MMP-13 by staining paraffin sections. Sample: Human breast carcinoma tissue. Primary antibody: Anti-MMP-13 (Ab-8) Rabbit pAb (Cat. No. PC542) (2.5 µg/ml). Detection: DAB, methyl green counterstain.
DescriptionPurified rabbit polyclonal antibody. Recognizes MMP-13.
BackgroundMMP-13 (~54 kDa), also known as collagenase-3, was originally identified in breast carcinomas but has since been shown to be present in high concentrations in other cancers, particularly in highly metastatic squamous-cell carcinomas of the head, neck, and vulva, in cutaneous basal-cell carcinomas, chondrosarcomas, melanomas, and in urinary bladder carcinomas. Expression of MMP-13 is not restricted to tumors and has been shown to also have a role in the repair of corneal wounding, and in the normal differentiation of osteoblasts. Mechanistically, MMP-13 appears to bind to a specific cell surface receptor and following interaction with the low-density lipoprotein receptor-related protein is internalized by the target cell. Like other members of the MMP family, MMP-13 has multiple substrate specificities including type II collagen, cartilage, fibronectin, and aggrecan.
HostRabbit
Immunogen speciesHuman
Immunogenrecombinant, human MMP-13
IsotypeIgG
Specieshuman, not mouse
Positive controlRecombinant MMP-13 or breast carcinoma tissue
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesBostrom, P.J., et al. 2000. Int. J. Cancer 88, 417.
Hofmann, U.B., et al. 2000. J. Invest. Dermatol. 115, 337.
Mengshol, J.A., et al. 2000. Arthritis Rheum. 43, 801.
Pendas, A.M., et al. 2000. Clin. Chim. Acta 291, 137.
Selvamurugan, N. and Partridge, N.C. 2000. Mol. Cell. Biol. Res. Commun. 3, 218.
Tuckermann, J.P., et al. 2000. J. Bone Miner. Res. 15, 1257.
Winchester, S.K., et al. 2000. J. Biol. Chem. 275, 23310.
Ye, H.Q., et al. 2000. Invest. Ophthalmol. Vis. Sci. 41, 2894.
Barmina, O.Y., et al. 1999. J. Biol. Chem. 274, 30087.
Botos, I., et al. 1999. J. Mol. Biol. 292, 837.
Winchester, S.K., et al. 1999. J. Cell Physiol. 181, 479.
Johansson, N., et al. 1997. Am. J. Pathol. 151, 499.