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MABN221 Anti-NCAM1 Antibody, clone 11G7.1

This NCAM1 antibody is validated for use in WB & IHC for the detection of the NCAM1 protein.

Anti-NCAM1 Antibody, clone 11G7.1 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: Ncam1, Ncam UniProt Number: P13595
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MABN221
100 µg  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN221
      DescriptionAnti-NCAM1 Antibody, clone 11G7.1
      Alternate Names
      • Neural cell adhesion molecule 1
      • N-CAM-1
      • NCAM-1
      • CD56
      Background InformationThe Immunoglobulin superfamily of cell adhesion molecules is related to the immunoglobulin proteins by structure. The family contains over a 100 members, which include ICAM (intercellular adhesion molecule), NCAM (neural cell adhesion molecule) and NgCAM (neural-glial cell adhesion molecule). Both NCAM and NgCAM are calcium independent adhesion molecules. NCAM, the first CAM to be characterized, is expressed on all neurons from very early in development. It binds homophilically to NCAM on adjacent cells and has a variety of functions during neural evelopment. One of these important functions is to hold developing neurites together during neuron outgrowth and formation of neural connections. The adhesive properties of NCAM are modulated during development and plasticity by the presence of the regulated carbohydrate, polysialic acid (PSA). The post-translational addition of alpha-2,8-linked PSA (NCAM-PSA) appears to contribute to synaptic plasticity during learning and memory formation and to the mechanisms of nerve regeneration.
      References
      Product Information
      FormatPurified
      Control
      • Rat brain tissue lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis NCAM1 antibody is validated for use in WB & IHC for the detection of the NCAM1 protein.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blot Analysis: 0.5 µg/mL from a representative lot detected NCAM1 in 10 µg of mouse brain tissue lysate.
      Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected NCAM1 in rat hippocampus and rat cerebral cortex tissues.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the cytoplasmic domain of mouse NCAM1.
      Epitopecytoplasmic domain
      Clone11G7.1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes the cytoplasmic domain of NCAM1.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Ncam1
      • Ncam
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~180 kDa observed. Uniprot describes 3 isoforms produced by alternative splicing at ~94 kDa, ~80 kDa, and ~67 kDa. The calculated molecular weight of this protein is 120 kDa, but can be observed between ~105-200 kDa due to high glycosylation (Patel, K., et al. (1991). Br J Cancer Suppl. 14:20-13).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in rat brain tissue lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected NCAM1 in 10 µg of rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABN221 04053252830570

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      Catégories

      Life Science Research > Antibodies and Assays > Primary Antibodies