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MABC1167-25UL Anti-P53DINP1 Antibody, clone F8

Detect using this rat monoclonal Anti-P53DINP1, clone F8 Antibody, Cat. No. MABC1167-25UL, validated for use in Western Blotting and ELISA.

Research Category: Apoptosis & Cancer Gene Symbol: TP53INP1, P53DINP1, SIP UniProt Number: Q96A56
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MABC1167-25UL
25 µL  
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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ELISARPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1167-25UL
      DescriptionAnti-P53DINP1 Antibody, clone F8
      Alternate Names
      • TP53INP1
      • Tumor protein p53-inducible nuclear protein 1
      • Stress-induced protein
      • p53-dependent damage-inducible nuclear protein 1
      Background InformationTumor protein p53-inducible nuclear protein 1 (UniProt: Q96A56; also known as Stress-induced protein, p53-dependent damage-inducible nuclear protein 1, p53DINP1) is encoded by the TP53INP1 (also known as P53DINP1, SIP) gene (GeneID: 94241) in human. p53DINP1 is a ubiquitously expressed protein with anti-proliferative and pro-apoptotic properties and is involved in cell stress response. It shuttles between the nucleus and the cytoplasm, depending on cellular stress conditions. It acts as a dual regulator of transcription and autophagy. p53DINP1 acts as a positive regulator of autophagy and in response to cellular stress or upon activation of autophagy, it relocates to autophagosomes where it interacts with autophagosome-associated proteins GABARAP, GABARAPL1/L2, MAP1LC3A/B/C to regulate autophagy. p53DINP1 also acts as an antioxidant and plays a major role in p53/TP53-driven oxidative stress response. It has both a p53/TP53-independent intracellular reactive oxygen species (ROS) regulatory function and a p53/TP53-dependent transcription regulatory function. p53DINP1 is reported to positively regulate p53/TP53 and p73/TP73 and stimulate their capacity to induce apoptosis. In response to double-strand DNA breaks, it promotes p53/TP53 phosphorylation on Ser46 and subsequent apoptosis. It is shown to acts as a tumor suppressor by inducing cell death by an autophagy and caspase-dependent mechanism.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect using this rat monoclonal Anti-P53DINP1, clone F8 Antibody, Cat. No. MABC1167-25UL, validated for use in Western Blotting and ELISA.
      Key Applications
      • Western Blotting
      • ELISA
      Application NotesWestern Blotting Analysis: A 1:62.5 dilution from a representative lot detected P53DINP1 in  10 µg of  human brain tumor, breast cancer, lymphoma, and pancreatic cancer tissue lysates.
      ELISA Analysis: A representative lot detected P53DINP1 in Elisa applications (Saadi, H., et. al. (2013). Results Immunol. 3:51-6).
      Western Blotting Analysis: A representative lot detected P53DINP1 in Western Blotting applications (Al Saati, T., et. al. (2013). Am J Pathol. 182(6):1996-2004; Peuget, S., et. al. (2014). Cell Death Differ. 21(7):1107-18; Saadi, H., et. al. (2013). Results Immunol. 3:51-6; Seillier, M., et. al. (2012). Cell Death Differ. 19(9):1525-35).
      Biological Information
      ImmunogenGST-tagged full length recombinant human Tumor protein p53-inducible nuclear protein 1.
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone F8 detects P53DINP1 (TP53INP1) protein in various human cancer cells.
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • TP53INP1
      • P53DINP1
      • SIP
      Purification MethodProtein G purified
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human colon tissue lysate.
      Western Blotting Analysis: A 1:62.5 dilution of this antibody detected P53DINP1 in  10 µg of human colon tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABC1167-25UL 04054839431753

      Documentation