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DR1028 Anti-PARC/H7-AP1 (900-950) Rabbit pAb

Anti-PARC/H7-AP1 (900-950) Rabbit pAb : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-p53 Associated Parkin-Like Cytoplasmic Protein, Anti-UbcH7 Associated Protein 1

DR1028
  
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      Aperçu

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      Description
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      Recognizes the ~270 kDa PARC/H7-AP1 protein in U20S cells.
      Catalogue NumberDR1028
      Brand Family Calbiochem®
      SynonymsAnti-p53 Associated Parkin-Like Cytoplasmic Protein, Anti-UbcH7 Associated Protein 1
      Application Data
      Lanes 1, 2: Detection of human PARC/H7-AP1 by immunoblotting (Lanes 1 and 2). Samples: Whole cell lysate (40 µg) from untreated U2OS cells (Lane 1) or U2OS cells that stably express a short hairpin RNA (shPARC) against PARC/H7-AP1 (Lane 2). Primary antibody: Anti-PARC/H7-AP1 (900-950) Rabbit pAb (Cat. No. DR1028) (1:2500). Detection: chemiluminescence. Lanes 3, 4: Detection of human PARC/H7-AP1 by immunoprecipitation followed by immunoblotting. Samples: Whole cell lysate (500 µg) from untreated U2OS cells. Antibodies for IP: Normal rabbit IgG (2 µg/mg lysate) (Lane 3) and Anti-PARC/H7-AP1 (Cat. No. DR1028) (2 µg/mg lysate) (Lane 4). Immunoblotting conditions: same as Lanes 1 and 2.
      References
      ReferencesNikolaev, A.Y., et al. Cell 112, 29.
      Product Information
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Positive controlU2OS cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:1000-1:5000)
      Immunoprecipitation (2-5 µg/mg lysate)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids 900-950 of human PARC
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Highly Toxic
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      DR1028 0

      Documentation

      Références bibliographiques

      Aperçu de la référence bibliographique
      Nikolaev, A.Y., et al. Cell 112, 29.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision23-August-2007 RFH
      SynonymsAnti-p53 Associated Parkin-Like Cytoplasmic Protein, Anti-UbcH7 Associated Protein 1
      ApplicationImmunoblotting (1:1000-1:5000)
      Immunoprecipitation (2-5 µg/mg lysate)
      Application Data
      Lanes 1, 2: Detection of human PARC/H7-AP1 by immunoblotting (Lanes 1 and 2). Samples: Whole cell lysate (40 µg) from untreated U2OS cells (Lane 1) or U2OS cells that stably express a short hairpin RNA (shPARC) against PARC/H7-AP1 (Lane 2). Primary antibody: Anti-PARC/H7-AP1 (900-950) Rabbit pAb (Cat. No. DR1028) (1:2500). Detection: chemiluminescence. Lanes 3, 4: Detection of human PARC/H7-AP1 by immunoprecipitation followed by immunoblotting. Samples: Whole cell lysate (500 µg) from untreated U2OS cells. Antibodies for IP: Normal rabbit IgG (2 µg/mg lysate) (Lane 3) and Anti-PARC/H7-AP1 (Cat. No. DR1028) (2 µg/mg lysate) (Lane 4). Immunoblotting conditions: same as Lanes 1 and 2.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~270 kDa PARC/H7-AP1 protein.
      Backgroundp53 is a tumor suppressor that can induce cell growth arrest, apoptosis, and cellular senescence in response to various types of cellular stress. Tight regulation of p53 is necessary for maintaining normal growth and tumor suppression. In unstressed cells wild-type p53 is a short-lived protein found at low levels. One mechanism for the regulation of p53 is compartmentalization. Upon exposure to DNA damaging agents or other cellular stress p53 translocates from the cytoplasm into the nucleus where it activates endogenous targets. PARC/H7-AP1 (Locus Link ID: 23113)is a Parkin-like ubiquitin ligase that serves as a cytoplasmic anchor for p53-associated protein complexes. PARC has been reported to form a large complex with p53 in the cytoplasm of unstressed cells. Inactivation of PARC/H7-AP1 induces p53 nuclear localization and apoptosis.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids 900-950 of human PARC
      IsotypeIgG
      Specieshuman, not mouse
      Positive controlU2OS cells
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
      Toxicity Highly Toxic
      ReferencesNikolaev, A.Y., et al. Cell 112, 29.