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AB5186-50UL Anti-Potassium Channel Kv2.1 Antibody

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AB5186-50UL
50 µL  
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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RIHC, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5186-50UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Potassium Channel Kv2.1 Antibody
      References
      Product Information
      FormatPurified
      Control
      • CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 50 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationPurified immunoglobulin by Protein A. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
      Quality LevelMQ100
      Applications
      ApplicationAnti-Potassium Channel Kv2.1 Antibody detects level of Potassium Channel Kv2.1 & has been published & validated for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot: 1:250-1:500 using ECL on rat brain membranes.

      Immunohistochemistry using rat brain sections.

      Dilutions should be made using a carrier protein such as BSA (1-3%)

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPurified peptide from rat Kv2.1 (amino acids 841-857) (Accession P15387),
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes a full length Kv2.1 protein. Does not cross react with any other potassium channel antigens tested so far.

      SPECIES REACTIVITIES: Should work in mouse (100% epitope homology) and in human and rabbit (88% epitope homology). Other species have not been tested.
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryVoltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shab-related subfamily. This member is a delayed rectifier potassium channel and its activity is modulated by some other family members.
      Gene Symbol
      • KCNB1
      • KV2.1
      • h-DRK1
      • Kv2.1
      • DRK1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q14721 # Mediates the voltage-dependent potassium ion permeability of excitable membranes. Channels open or close in response to the voltage difference across the membrane, letting potassium ions pass in accordance with their electrochemical gradient.
      SIZE: 858 amino acids; 95878 Da
      SUBUNIT: Heteromultimer with KCNG2, KCNG3, KCNG4, KCNS1, KCNS2, KCNS3 and KCNV2 (By similarity).
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      DOMAIN: SwissProt: Q14721 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. & The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
      PTM: Phosphorylated on serine residues in the C-terminal cytoplasmic tail (By similarity).
      SIMILARITY: Belongs to the potassium channel family. B (Shab) subfamily.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      AB5186-50UL 04053252734274

      Documentation

      Anti-Potassium Channel Kv2.1 Antibody FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-Potassium Channel Kv2.1 Antibody Certificats d'analyse

      TitreNuméro de lot
      RABBIT ANTI-Kv2.1 2463617
      RABBIT ANTI-Kv2.1 - 3574386 3574386
      RABBIT ANTI-Kv2.1 - 4256282 4256282
      RABBIT ANTI-Kv2.1 PURIFIED POLYCLONAL ANTIBODY 3005360

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
      Short-term effects of rapid pacing on mRNA level of voltage-dependent K(+) channels in rat atrium: electrical remodeling in paroxysmal atrial tachycardia.
      Yamashita, T, et al.
      Circulation, 101: 2007-14 (2000)  1999

      Afficher le résumé
      10779469 10779469
      Potassium (K+) channel expression in basal forebrain cholinergic neurons.
      Betancourt, L and Colom, L V
      J. Neurosci. Res., 61: 646-51 (2000)  1999

      10972961 10972961

      Fiche technique

      Titre
      RABBIT ANTI-Kv2.1 PURIFIED POLYCLONAL ANTIBODY