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ST1702 Anti-RAG2 Mouse mAb (2G8)

Anti-RAG2 Mouse mAb (2G8) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-Recombinant Activating Gene 2

ST1702
  
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      Recognizes the RAG2 protein.
      Catalogue NumberST1702
      Brand Family Calbiochem®
      SynonymsAnti-Recombinant Activating Gene 2
      Application Data
      Detection of human RAG2 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-RAG2 Mouse mAb (2G8) (Cat. No. ST1702) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      Formliquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see coments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 428-528 human RAG2, expressed as a GST fusion protein
      Clone2G8
      HostMouse
      IsotypeIgG2b
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      ST1702 0

      Documentation

      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-November-2010 RFH
      SynonymsAnti-Recombinant Activating Gene 2
      ApplicationELISA (see coments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human RAG2 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-RAG2 Mouse mAb (2G8) (Cat. No. ST1702) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the RAG2 protein.
      Backgroundhis gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 428-528 human RAG2, expressed as a GST fusion protein
      Clone2G8
      IsotypeIgG2b
      Specieshuman
      Formliquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review