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MABC1640-25UL Anti-RIPK3/RIP3 Antibody, clone 1H2

Anti-RIPK3/RIP3, clone 1H2, Cat. No. MABC1640, is a rat monoclonal antibody that detects RIPK3/RIP3 and is tested for use in Immunofluorescence and Western Blotting.

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MABC1640-25UL
25 µL  
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      Replacement Information
      Description
      Catalogue NumberMABC1640-25UL
      DescriptionAnti-RIPK3/RIP3 Antibody, clone 1H2
      Alternate Names
      • Receptor-interacting serine/threonine-protein kinase 3
      • EC:2.7.11.1
      • RIP-like protein kinase 3
      • Receptor-interacting protein 3
      • RIP-3
      Background InformationReceptor-interacting serine/threonine-protein kinase 3 (UniProt: Q9Y572; also known as EC:2.7.11.1, RIP-like protein kinase 3, Receptor-interacting protein 3, RIP-3) is encoded by the RIPK3 (also known as RIP3) gene (Gene ID: 11035) in human. RIPK3 is a serine/threonine protein kinase that is expressed in embryo and adult spleen, liver, testis, brain, and lung. Its protein kinase domain is localized in amino acids 21 to 287 in the N-terminal half and it also has a C-terminal RIP homolytic interaction motif (RHIM; aa 450-466), which mediates interaction with the RHIM motif of RIPK1 and facilitate the formation of a necrosome. RIPK3 is shown to activate both necroptosis and apoptosis. Activated RIPK3 forms a necrosis-inducing complex and mediates phosphorylation of MLKL, promoting MLKL localization to the plasma membrane and execution of programmed necrosis that is characterized by calcium influx and plasma membrane damage. Its location is mainly cytoplasmic, however, following infection, it can also be detected in the nucleus. RIPK3 also regulates apoptosis that is dependent on RIPK1, FADD, and CASP8, and is independent of MLKL and RIPK3 kinase activity. It phosphorylates RIPK1 and then RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation. In some cell types, RIPK3 is also reported to restrict viral replication by promoting cell death-independent responses. Clone 8G7 is raised against an extended kinase domain (residues 2-353) of mouse RIPK3 and can detect forms that may not be detected by antibodies directed towards the C-terminus of the full-length RIPK3. (Ref.: Samson, AL., et al. (2021). Cell Death Differ. https://doi.org/10.1038/s41418-021-00742-x; Petrie, EJ., et al. (2019). Cell Rep. 28(13); 3309-3319).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-RIPK3/RIP3, clone 1H2, Cat. No. MABC1640, is a rat monoclonal antibody that detects RIPK3/RIP3 and is tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected RIPK3/RIP3 in Western Blotting applications (Samson, A.L., et. al. (2020). Nat Commun. 11(1):3151).

      Immunofluorescence Analysis: A representative lot detected RIPK3/RIP3 in Immunofluorescence applications (Samson, A.L., et. al. (2021). Cell Death Differ. https://doi.org/10.1038/s41418-021-00742-x).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenRecombinant fragment corresponding to 355 amino acids from the extended kinase domain of human Receptor-interacting serine/threonine-protein kinase 3 (RIPK3).
      EpitopeUnknown
      Clone1H2
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone 1H2 is a rat monoclonal antibody that detects human Receptor-interacting serine/threonine-protein kinase (RIP3). It targets an epitope within the extended kinase domain that includes most of the N-terminal half and a part of C-terminal half.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RIPK3
      • RIP3
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~57 kDa observed; 56.89 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HT-29 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected RIPK3/RIP3 in HT-29 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABC1640-25UL 04065266553680