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ABC1454-25UL Anti-Sodium-Iodide Symporter Antibody, C-terminal 2

Detect Sodium/iodide cotransporter using this rabbit polyclonal Anti-Sodium-Iodide Symporter, C-terminal 2, Cat. No. ABC1454-25UL, validated for use in Immunohistochemistry (Paraffin), Immunocytochemistry and Western Blotting,

Research Category: Apoptosis & Cancer Gene Symbol: SLC5A5, NIS UniProt Number: Q92911
ABC1454-25UL
25 µL  
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      Replacement Information

      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P), ICC, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC1454-25UL
      DescriptionAnti-Sodium-Iodide Symporter Antibody, C-terminal 2
      Alternate Names
      • Sodium/iodide cotransporter
      • Na(+)/I(-) cotransporter
      • Na(+)/I(-) symporter
      Background InformationSodium/iodide cotransporter (UniProt: Q92911; also known as Na(+)/I(-) cotransporter, Sodium-iodide symporter, Na(+)/I(-) symporter) is encoded by the SLC5A5 (also known as NIS) gene (Gene ID: 6528) in human. Sodium/iodide cotransporter is a multi-pass membrane protein that mediates iodide uptake in thyroid gland. It is expressed primarily in thyroid tissue, but is present in lower levels in mammary gland and ovary. It expression is significantly reduced in tumors. The presence of symporter in mammary glands leads to secretion of iodine in milk that is required for thyroid function in neonatal animals. NIS gene and protein expression is stimulated by thyroid-stimulating hormone. Both human and rat NIS contain N-glycosylation sites, however, glycosylation is not required for its full activity. Abnormalities in the expression of NIS gene lead to thyroid disease and can result in congenital hypothyroidism. Autoantibodies to NIS adversely affect iodide transport.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing  76 mM Tris, 192 mM glycine, pH 7.4   with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Sodium/iodide cotransporter using this rabbit polyclonal Anti-Sodium-Iodide Symporter, C-terminal 2, Cat. No. ABC1454-25UL, validated for use in Immunohistochemistry (Paraffin), Immunocytochemistry and Western Blotting,
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Sodium-Iodide Symporter in human thyroid and human salivary gland tissue sections.
      Immunocytochemistry Analysis: A representative lot detected Sodium-Iodide Symporter in COS-7 cells transfected with wild-type human NIS and in COS-7 cells transfected with mutant Q267E human NIS (De la Vieja, A., et. al. (2005). Mol Endocrinol. 19(11):2847-58; De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87).
      Immunohistochemistry Analysis: A representative lot detected Sodium-Iodide Symporter in Immunohistochemistry application (De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87; Wapnir, I.L., et. al. (2004). Clin Cancer Res. 10(13):4294-302; Wapnir, I.L., et. al. (2003). J Clin Endocrinol Metab. 88(4):1880-8).
      Immunoprecipitation Analysis: A representative lot detected Sodium-Iodide Symporter in Biotinylated NIS constructs expressed in COS-7 cells (De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87).
      Flow Cytometry Analysis: A representative lot detected Sodium-Iodide Symporter in COS-7 cells transfected with WT and mutant Q267E NIS (De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87).
      Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Sodium-Iodide Symporter in COS-7 cells transfected with hNIS ( Courtesy of Dr Nancy Carrasco & Dr. Andrea Reyna-Neyra  at Yale University ).
      Western Blotting Analysis: A representative lot detected Sodium-Iodide Symporter in COS-7 cells transfected with NIS and COS-7 cells transfected with WT and mutant Q267E NIS (De la Vieja, A., et. al. (2005). Mol Endocrinol. 19(11):2847-58; De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87).
      Immunohistochemistry Analysis: A representative lot detected Sodium-Iodide Symporter in IHC-P human salivary gland and breast tissues (Tazebay, U.H., et. al. (2000). Nat Med. 6(8):871-8).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of human Sodium-iodide symporter.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Sodium-iodide symporter in human. It targets an epitope within 13 amino acids from the C-terminal end. For Western blotting application, please do not boil the sample to avoid any aggregation. When transfering protein to nitrocellulose membrane use of 0.05% (w/v) lithium dodecyl sufate is highly recommended in the transfer buffer.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • SLC5A5
      • NIS
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight68.6 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human stomach tissue sections.

      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Sodium-Iodide Symporter in human stomach tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      ABC1454-25UL 04054839441615

      Documentation