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05-1116 Anti-VEGF Antibody, clone CH-10

Anti-VEGF Antibody, clone CH-10 is an antibody against VEGF for use in WB.

Anti-VEGF Antibody, clone CH-10 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Signaling Research Sub-Category: Growth Factors & Receptors Gene Symbol: MGC70609, MVCD1, VEGF, VEGF-A, VPF UniProt Number: P15692
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05-1116
100 µg  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWBMAffinity PurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1116
      ReplacesMAB1665
      DescriptionAnti-VEGF Antibody, clone CH-10
      Alternate Names
      • Vascular permeability factor
      • vascular endothelial growth factor
      • vascular endothelial growth factor A
      • vascular endothelial growth factor isoform VEGF165
      Background InformationVEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic mitogens. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Jurkat cell lysate
      PresentationLiquid in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6 containing 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-VEGF Antibody, clone CH-10 is an antibody against VEGF for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenRecombinant mouse VEGF.
      CloneCH-10
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes human and rat VEGF, other variants not tested.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms.
      Gene Symbol
      • MGC70609
      • MVCD1
      • VEGF
      • VEGF-A
      • VPF
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to VEGFR2/Kdr but doesn't activate downstream signaling pathways, doesn't activate angiogenesis and inhibits tumor growth.
      SUBUNIT STRUCTURE: Homodimer; disulfide-linked. Also found as heterodimer with PlGF By similarity.
      SUBCELLULAR LOCATION: Secreted. Note: VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
      TISSUE SPECIFICITY: The VEGF189, VEGF-165 and VEGF-121 isoforms are widely expressed, whereas the VEGF206 and VEGF-145 are uncommon.
      INDUCTION: Regulated by growth factors, cytokines, gonadotropins, nitric oxide, hypoxia, hypoglycemia and oncogenic mutations.
      SEQUENCE SIMILARITIES: Belongs to the PDGF/VEGF growth factor family.
      SEQUENCE CAUTION: The sequence CAC19512.2 differs from that shown. Reason: Erroneous gene model prediction.
      Molecular Weight12/16/19 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Jurkat cell lysate.
      Western Blot Analysis: 1:1000 dilution of this antibody detected VEGF on 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      05-1116 04053252278877

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      Catégories

      Life Science Research > Antibodies and Assays > Primary Antibodies