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07-441-AF488 Anti-dimethyl-Histone H3 (Lys9) Antibody, AlexaFluor® 488 Conjugate

Anti-dimethyl-Histone H3 (Lys9) Antibody, AlexaFluor® 488 Conjugate is an antibody against dimethyl-Histone H3 (Lys9) Antibody for use in Immunocytochemistry.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: HIST3H3, H3FT UniProt Number: Q16695
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07-441-AF488
100 µL  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICCRbAlexaFluor®488Polyclonal Antibody
      Description
      Catalogue Number07-441-AF488
      DescriptionAnti-dimethyl-Histone H3 (Lys9) Antibody, AlexaFluor® 488 Conjugate
      Alternate Names
      • Histone H3.1t
      • H3/t
      • H3t
      • H3/g
      • dimethyl-Histone H3 (Lys9), AlexaFluor® 488 Conjugate
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
      References
      Product Information
      FormatAlexaFluor®488
      PresentationPurified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1 % sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-dimethyl-Histone H3 (Lys9) Antibody, AlexaFluor® 488 Conjugate is an antibody against dimethyl-Histone H3 (Lys9) Antibody for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected dimethyl-Histone H3 (Lys9) in HepG2 cells.
      The unconjugated antibody (Cat. No. 07-441) is shown to be suitable also for Western blotting, dot blot, immunocytochemistry, and peptide inhibition assay (peptide blocking) applications.
      Biological Information
      ImmunogenKLH-conjugated, 2X-branched synthetic peptide containing the sequence ..RKme2STG.., in which Kme2 corresponds to dimethyl-lysine at residue 9 of human Histone H3.
      EpitopeH3K9me2
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityHistone H3 dimethylated on lysine 9.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Yeast, Rat, Chicken based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST3H3
      • H3FT
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~17 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected dimethyl-Histone H3 (Lys9) in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      07-441-AF488 04055977294798

      Documentation

      Anti-dimethyl-Histone H3 (Lys9) Antibody, AlexaFluor® 488 Conjugate FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-dimethyl-Histone H3 (Lys9) Antibody, AlexaFluor® 488 Conjugate Certificats d'analyse

      TitreNuméro de lot
      Anti-dimethyl-Histone H3 (Lys9), AlexaFluor® 488 Conjugate 2868340
      Anti-dimethyl-Histone H3 (Lys9), AlexaFluor® 488 Conjugate - 3198393 3198393
      Anti-dimethyl-Histone H3 (Lys9), AlexaFluor® 488 Conjugate - 4133766 4133766
      Anti-dimethyl-Histone H3 (Lys9), AlexaFluor® 488 Conjugate -Q2611355 Q2611355

      Brochure

      Titre
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      Posters

      Titre
      Post-Translational Modifications