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SCC162 CFTE29o- Human Cystic Fibrosis Tracheal Epithelial Cell Line

CFTE29o- human cystic fibrosis tracheal epithelial cell line is a useful model for cystic fibrosis research and for studying chloride ion transport and ion transport in human airways.

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Key Applications: Cell Culture, Cell Based Assays
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SCC162
≥1X10⁶ cells/vial   
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      Key Applications
      CULT, Cell Based Assays
      Description
      Catalogue NumberSCC162
      DescriptionCFTE29o- Human Cystic Fibrosis Tracheal Epithelial Cell Line
      Alternate Names
      • CFTE29o
      • CFTE-29o-
      Background InformationCystic Fibrosis (CF) is a lethal autosomal recessive disease caused by mutations in the CF transmembrane conductance regulator (CFTR) gene which functions as a cAMP-activated and phosphorylated-regulated Cl channel. The predominant mutation in the CFTR gene is a trinucleotide deletion that results in loss of a phenylalanine at amino acids 508 (ΔF508) in the CFTR protein. This mutation accounts for ~66% of all CF alleles (1).

      CFTE29o- is a human CF tracheal epithelial cell line, derived from a 21-yr old male CF patient homozygous for the ΔF508 CFTR mutation and immortalized with the origin-of-replication defective SV40 plasmid (pSVori-) (1). The cell line express keratin, indicating an epithelial cell origin, along with a calcium-dependent cell adhesion protein, cellCAM 120/80 (1), and ZO-1 indicating the ability to form tight junctions. CFTE29o- displays ion transport properties characteristic of cystic fibrosis such as defective cAMP-dependent chloride transport. Upon treatment with the calcium ionophore, ionomycin, CFTE29o- secretes chloride, albeit at reduced levels relative to normal cells. The cells express CFTR mRNA, and small amounts of CFTR protein as assessed by Western blot hybridization (1). Karyotype analysis indicates that 70% of the cells contain two copies of chromosome 7 (1).

      CFTE29o- is a useful model for cystic fibrosis and for studying chloride ion transport and ion transport in human airways. The cells are useful for testing candidate chemotherapeutic drugs for their potential as treatments for ameliorating CF pathologies.

      References:
      1. Kunzelmann K, Schwiebert EM, Zeitlin PL, Kuo WL, Stanton BA, Gruenert DC. (1993) An immortalized cystic fibrosis tracheal epithelial cell line homozygous for the delta F508 CFTR mutation. Am J Respir Cell Mol Bio 8(5): 522-529.
      References
      Product Information
      Applications
      ApplicationCFTE29o- human cystic fibrosis tracheal epithelial cell line is a useful model for cystic fibrosis research and for studying chloride ion transport and ion transport in human airways.
      Key Applications
      • Cell Culture
      • Cell Based Assays
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Epithelial Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
      • Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, C, and HIV-1 & 2 viruses as assessed by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are negative for mycoplasma contamination.
      • Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      SCC162 04054839464904