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AAMT005 Mitochondrial Complex V Activity Assay Kit

Mitochondrial Complex V Activity Assay Kit : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
AAMT005
  
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      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Product Description

      The ATP synthase complex (EC 3.6.3.14), also called Complex V or F1F0 ATPase, is responsible for ATP production in the oxidative phosphorylation process and can work in reverse as a proton pumping ATPase. The enzyme was thought to be localized exclusively in the mitochondria, but it has now also been identified on the plasma membrane of several cell types, including hepatocytes (where it acts as an HDL receptor), on endothelial cells (where it may act as an angiostatin receptor), and on the surface of cancer cells.

      The mammalian ATP synthase is composed of 17 subunits. Five of the subunits make up the easily detached F1 domain. The remainder subunits are part of two stalk domains and the proton pumping F0 domain. Two of the F0 subunits are encoded on mitochondrial (mt) DNA while all other subunits of the ATP synthase complex are nuclear encoded. Mutations in the mt-encoded subunits are implicated in OXPHOS disease.

      The Mitochondrial Complex V (ATP Synthase) Activity Assay Kit is used to determine the activity of this enzyme in a sample. ATP Synthase is immunocaptured within the wells. The enzyme functions by hydrolyzing ATP to ADP and phosphate. This production of ADP is ultimately coupled, as shown below, to the oxidation of NADH to NAD+ which is monitored as a decrease in absorbance at 340 nm (See References section for an article that describes the nature of this assay). The ATP hydrolysis activity and therefore the coupled reaction is inhibited by oligomycin (a specific inhibitor of ATP Synthase). See Figure 1 at the bottom of this page for an overview of the overall reaction.

      PK- Pyruvate kinase, LDH-Lactate dehydrogenase, PEP-phosphoenolpyruvate

      Panel Components

      10 ml 	Buffer, (72566-10ML)
1 ml	Detergent, (72567-1ML)
20 ml	Reagent Mix, (72568-20ML)
6 ml	Phospholipids, (72569-6ML)
1 ea	Microplate, (72574-1EA)

      * This kit contains sufficient materials for 96 measurements (one 96-well microplate).



      Storage: Store Buffer, Detergent, and covered Microplate at 2 to 8°C. Store the Phospholipids and Reagent Mix at –80°C. For multiple experiments, it is recommened to aliquot the Reagent Mix and Phospholipids upon initial thaw and store any aliquots at
      -80°C.

      The Mitochondrial Complex V (ATP Synthase) Activity Assay Kit contains sufficient materials for 96 measurements (one 96-well microplate).The 96-well microplate in this kit has a monoclonal antibody pre-bound to the wells. This plate can be broken into 12 separate
      8-well strips; therefore the plate can be used for up to 12 separate experiments.
      Catalogue NumberAAMT005
      Brand Family Novagen®
      References
      Product Information
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Multiple Storage Conditions
      Toxicity Irritant
      Storage Multiple storage requirements
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      AAMT005 0