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DR1002 PhosphoDetect™ Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12)

This PhosphoDetect™ Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12) is validated for use in Immunoblotting, Immunocytochemistry for the detection of ATM (pSer¹⁹⁸¹).

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-Ataxia Telangiectasia, Phospho-Specific (Ser¹⁹⁸¹)

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DR1002
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Aperçu

Replacement Information

Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~370 kDa ATM protein phosphorylated on Ser1981 in camptothecin-treated HeLa cells. Sold under license of U.S. Patent 7,108,992.
Catalogue NumberDR1002
Brand Family Calbiochem®
SynonymsAnti-Ataxia Telangiectasia, Phospho-Specific (Ser¹⁹⁸¹)
Application Data
Detection of human ATM phosphorylated at Ser1981 by immunoblotting. Samples: Whole cell lysate (50 µg) from untreated HeLa cells (left lane) and camptothecin-treated (100 µM) HeLa cells (right lane). Primary antibody: PhosphoDetect™ Anti-ATM (pSer1981) Mouse mAb (10H11.E12) (Cat. No. DR1002) (0.5 µg/ml). Detection: chemiluminescence.

Detection of human ATM phosphorylated at (Ser1981) by immunofluorescence. Samples: Untreated HeLa cells (left panel) and camptothecin-treated (10 µM, 2 h) HeLa cells, fixed with 100% methanol (right panel). Primary antibody: PhosphoDetect™ Anti-ATM (pSer1981) Mouse mAb (10H11.E12) (Cat. No. DR1002) (1.5 µg/ml). Detection: fluorescence.
References
ReferencesHorejsi, Z., et al. 2004. Oncogene 23, 3122.
Abraham, R.T. 2003. Genes Dev. 15, 2177.
Bakkenist, C.J. and Kastan, M.B. 2003. Nature 421, 499.
Shiloh, Y. 2003. Nat. Rev. Cancer 3, 155.
Product Information
DeclarationSold under license of U.S. Patent 7,108,992.
FormLiquid
FormulationIn PBS.
Positive controlHeLa cells treated with camptothecin (Cat. No. 208925)
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesImmunoblotting (0.5 µg/ml)
Immunocytochemistry (1.5 µg/ml)
Application CommentsDoes not detect the unphosphorylated protein. Use 5% milk in TBS-T for blocking; the use of BSA is not recommended. The use of PBS-T is not recommended, as phosphate-containing buffers can contribute to high background with phosphospecific antibodies. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide (SLAFEEGpSQSTTISS) corresponding to amino acids 1974-1988 surrounding the Ser¹⁹⁸¹ phosphorylation site of human ATM
ImmunogenHuman
Clone10H11.E12
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Irritant
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

PhosphoDetect™ Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12) FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

PhosphoDetect™ Anti-ATM (pSer¹⁹⁸¹) Mouse mAb (10H11.E12) Certificats d'analyse

TitreNuméro de lot
DR1002

Références bibliographiques

Aperçu de la référence bibliographique
Horejsi, Z., et al. 2004. Oncogene 23, 3122.
Abraham, R.T. 2003. Genes Dev. 15, 2177.
Bakkenist, C.J. and Kastan, M.B. 2003. Nature 421, 499.
Shiloh, Y. 2003. Nat. Rev. Cancer 3, 155.
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-August-2007 RFH
SynonymsAnti-Ataxia Telangiectasia, Phospho-Specific (Ser¹⁹⁸¹)
ApplicationImmunoblotting (0.5 µg/ml)
Immunocytochemistry (1.5 µg/ml)
Application Data
Detection of human ATM phosphorylated at Ser1981 by immunoblotting. Samples: Whole cell lysate (50 µg) from untreated HeLa cells (left lane) and camptothecin-treated (100 µM) HeLa cells (right lane). Primary antibody: PhosphoDetect™ Anti-ATM (pSer1981) Mouse mAb (10H11.E12) (Cat. No. DR1002) (0.5 µg/ml). Detection: chemiluminescence.

Detection of human ATM phosphorylated at (Ser1981) by immunofluorescence. Samples: Untreated HeLa cells (left panel) and camptothecin-treated (10 µM, 2 h) HeLa cells, fixed with 100% methanol (right panel). Primary antibody: PhosphoDetect™ Anti-ATM (pSer1981) Mouse mAb (10H11.E12) (Cat. No. DR1002) (1.5 µg/ml). Detection: fluorescence.
DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~370 kDa ATM protein phosphorylated at Ser1981.
BackgroundATM (ataxia telangiectasia mutated, ACC# NP_000042) is a member of the PI-3/PI-4 kinase family and is a central mediator of the cellular response to DNA damage and cellular stress. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. ATM is a regulator of numerous downstream proteins, including the tumor suppressor proteins, p53 and BRCA1, the checkpoint kinase, CHK2, the checkpoint proteins RAD17 and RAD9, and the DNA repair protein NBS1. ATM and the functionally related ATR kinase are thought to be master regulators of cell cycle checkpoint signaling pathways that are required for the cellular response to DNA damage and as well as genome stability. Activation of ATM in response to DNA damage requires intermolecular autophosphorylation of Ser1981 and dimer dissociation.
HostMouse
Immunogen speciesHuman
Immunogena synthetic phosphopeptide (SLAFEEGpSQSTTISS) corresponding to amino acids 1974-1988 surrounding the Ser¹⁹⁸¹ phosphorylation site of human ATM
Clone10H11.E12
IsotypeIgG₁
Specieshuman, mouse
Positive controlHeLa cells treated with camptothecin (Cat. No. 208925)
FormLiquid
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsDoes not detect the unphosphorylated protein. Use 5% milk in TBS-T for blocking; the use of BSA is not recommended. The use of PBS-T is not recommended, as phosphate-containing buffers can contribute to high background with phosphospecific antibodies. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Irritant
ReferencesHorejsi, Z., et al. 2004. Oncogene 23, 3122.
Abraham, R.T. 2003. Genes Dev. 15, 2177.
Bakkenist, C.J. and Kastan, M.B. 2003. Nature 421, 499.
Shiloh, Y. 2003. Nat. Rev. Cancer 3, 155.