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525189 Phospholipase C-γ-2, His•Tag®, Human, Recombinant, S. frugiperda

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Phospholipase C-γ-2, Human, Recombinant

525189
  
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      Aperçu

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Full-length, recombinant, human phospholipase C γ-2 fused at the N-terminus to His•Tag® and S•Tag™ sequences and expressed in S. frugiperda insect cells. This preparation is qualified for use as a substrate for protein tyrosine kinases in in vitro assays. Intrinsic enzyme activity has not been tested. Production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes, which are crucial enzymes in transmembrane signaling. PLC-γ-2 is phosphorylated on tyrosine residues following ligand-induced activation of a variety of growth factor receptors and immune system receptors, resulting in increased phospholipase activity. The full-length proteins contains the following domains: C2 domain (aa 1059-1152); PH domain (aa 20-131); PI-PLC X-box catalytic domain (aa 312-456); PI-PLC Y-box catalytic domain (aa 930-1044); two SH2 domains (aa 532-635, aa 646-735); and a SH3 domain (aa 769-829). It has been reported to be phosphorylated at Tyr753 and Tyr759 (Btk, Src, Fyn, Lck), and Tyr1217 and Tyr1245 by Bcr-Abl.
      Catalogue Number525189
      Brand Family Calbiochem®
      SynonymsPhospholipase C-γ-2, Human, Recombinant
      Application Data
      References
      ReferencesRodriguez, R., et al. 2001. J. Biol. Chem. 276, 47982.
      Lennon, G., et al. 1996. Genomics 33, 151.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Irritant
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      525189 0

      Documentation

      Références bibliographiques

      Aperçu de la référence bibliographique
      Rodriguez, R., et al. 2001. J. Biol. Chem. 276, 47982.
      Lennon, G., et al. 1996. Genomics 33, 151.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision26-August-2008 RFH
      SynonymsPhospholipase C-γ-2, Human, Recombinant
      Application Data
      DescriptionFull-length, recombinant, human phospholipase C γ-2 fused at the N-terminus to His•Tag® and S•Tag™ sequences and expressed in S. frugiperda insect cells. This preparation is qualified for use as a substrate for protein tyrosine kinases in in vitro assays. Intrinsic enzyme activity has not been tested. Production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes, which are crucial enzymes in transmembrane signaling. PLC-γ-2 is phosphorylated on tyrosine residues following ligand-induced activation of a variety of growth factor receptors and immune system receptors, resulting in increased phospholipase activity. The full-length proteins contains the following domains: C2 domain (aa 1059-1152); PH domain (aa 20-131); PI-PLC X-box catalytic domain (aa 312-456); PI-PLC Y-box catalytic domain (aa 930-1044); two SH2 domains (aa 532-635, aa 646-735); and a SH3 domain (aa 769-829). It has been reported to be phosphorylated at Tyr753 and Tyr759 (Btk, Src, Fyn, Lck), and Tyr1217 and Tyr1245 by Bcr-Abl.
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥90% by SDS-PAGE
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Irritant
      ReferencesRodriguez, R., et al. 2001. J. Biol. Chem. 276, 47982.
      Lennon, G., et al. 1996. Genomics 33, 151.