Millipore Sigma Vibrant Logo

539251 Protein A, Alkaline Phosphatase Conjugate

This Protein A, Alkaline Phosphatase Conjugate is validated for use in Direct Immunoassay.

Protein A, Alkaline Phosphatase Conjugate : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
View Products on Sigmaaldrich.com
539251
Voir les Prix & la Disponibilité

Aperçu

Replacement Information

Prix & Disponibilité

Référence DisponibilitéConditionnement Qté Prix Quantité
539251-1MG
Récupération des données relatives à la disponibilité...
—
Disponibilité limitée Disponibilité limitée
En stock 
Interrompu(e)
Disponible en quantités limitées
Disponibilité à confirmer
    Pour le restant : Nous vous tiendrons informé
      Pour le restant : Nous vous tiendrons informé
      Nous vous tiendrons informé
      Contacter le Service Clients
      Contact Customer Service

      		 Ampoule plast. 1 mg
      Prix en cours de récupération
      Le prix n'a pas pu être récupéré
      La quantité minimale doit être un multiple de
      Maximum Quantity is
      À la validation de la commande Plus d'informations
      Vous avez sauvegardé ()
       
      —
      Demander le prix
      		 —
      Description
      OverviewNative protein A conjugated to alkaline phosphatase. Can be used instead of an alkaline phosphatase-conjugated secondary antibody in many immunological assays.
      Catalogue Number539251
      Brand Family Calbiochem®
      References
      Product Information
      FormLiquid
      FormulationIn 50 mM Tris, 1 mM MgCl₂, 1% BSA, pH 8.0.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesDirect Immunoassay (1:5000)
      Application Commentsp-Nitrophenyl phosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate for testing immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      PuritySingle band by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      539251-1MG 04055977268980

      Documentation

      Protein A, Alkaline Phosphatase Conjugate FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Protein A, Alkaline Phosphatase Conjugate Certificats d'analyse

      TitreNuméro de lot
      539251
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-August-2007 RFH
      ApplicationDirect Immunoassay (1:5000)
      DescriptionNative protein A from Staphylococcus aureus conjugated to alkaline phosphatase.
      BackgroundProtein A is the universal "second antibody" because it binds to the Fc portions of most immunoglobulin classes. It is a versatile and economic means to isolate, detect, and purify antibodies produced by many animals. It is especially useful in immunohistochemical studies because, unlike secondary antibodies, it does not bind to cell Fc receptors, and therefore will not give high background reactions. Binding strength is as follows: strongly reactive: guinea pig, human IgG1, IgG2, and IgG4, and rabbit; weakly reactive: bovine, Human IgA, IgM, and IgE, and mouse; nonreactive: goat, horse, human IgD and IgG3, rat, and sheep.
      FormLiquid
      FormulationIn 50 mM Tris, 1 mM MgCl₂, 1% BSA, pH 8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      PuritySingle band by SDS-PAGE
      Preservative≤0.1% sodium azide
      Commentsp-Nitrophenyl phosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate for testing immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling