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539723 ProteoExtract® Phosphopeptide Enrichment SCIMAC Kit

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539723
  
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      Replacement Information
      Description
      Overview

      This product has been discontinued.






      The ProteoExtract® Phosphopeptide Enrichment SCIMAC Kit is a useful tool for enrichment of phosphorylated peptides from complex samples by combination of strong cation exchange chromatography (SCX) and immobilized metal ion affinity chromatography (IMAC). The identification of phosphorylation sites is routinely accomplished by mass spectrometry. Captured and eluted phosphopeptide fractions are ready for MALDI- and ESI- mass spectrometry analysis.
      Catalogue Number539723
      Brand Family Calbiochem®
      Application Data
      The combination of SCX and IMAC exhibits high selectivity for phosphopeptides by reducing nonspecific binding of peptides from complex mixtures. A complex peptide mixture derived from a tryptic digest of porcine liver extract was spiked with α-casein and two synthetic phosphopeptides. The samples were subsequently processed using (A) an ironoxide-based IMAC material alone or (B) the combination of SCX with zirconium-based IMAC particles as outlined in the Detailed Protocol. Mass spectrometry analysis was performed using ESI-LC/MS equipment operated in positive mode.
      Materials Required but Not Delivered• Micropipettes and tips; 10 µl, 200 µl, and 1 ml
      • Microcentrifuge and rotor for >10,000 x g
      • 1.5 ml microcentrifuge tubes
      • Magnetic stand or separator (optional)
      References
      Product Information
      Detection methodMass Spectrometry
      Form100 Isolations
      FormatPhosphopeptide enrichment
      Kit contains10X Phosphobind Buffer, Wash Buffer 1, Wash Buffer 2, Elution Buffer, MagPrep® Phosphobind Particles, SCX Resin, SCX Elute Buffer, and a user protocol.
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Intended useThe ProteoExtract® Phosphopeptide Enrichment SCIMAC Kit is designed to enrich phosphorylated peptides from complex samples using a combination of strong cation exchange chromatography (SCX) and immobilized metal ion affinity chromatography (IMAC) The final phosphopeptide fraction is directly compatible with downstream analysis by MALDI- and ESI mass spectrometry.
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage +2°C to +8°C
      Storage ConditionsUpon arrival store the entire contents of the kit at 4°C for long-term storage or at room temperature for up to 6 months.
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Kit contains10X Phosphobind Buffer, Wash Buffer 1, Wash Buffer 2, Elution Buffer, MagPrep® Phosphobind Particles, SCX Resin, SCX Elute Buffer, and a user protocol.
      Specifications
      Global Trade Item Number
      Référence GTIN
      539723 0