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SAE0108-100UG SAvPhire Monomeric Streptavidin peroxidase conjugate

SAvPhire Monomeric Streptavidin,Cat.#SAE0108, is a high-affinity recombinant variant of SA that minimizes the risk of aggregation, precipitation, and crosslinking in pre-incubation with biotinylated molecules and offers easier access to the desired targets, tested for use in IHC (Paraffin) & WB.

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Species Reactivity: All
SAE0108-100UG
100 μg  
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      Replacement Information
      Description
      Catalogue NumberSAE0108-100UG
      DescriptionSAvPhire Monomeric Streptavidin peroxidase conjugate
      Alternate Names
      • SAVphire-HRP
      • mSA-HRP
      Background InformationStreptavidin, a tetrameric protein, has been extensively used in purification and detection of biomolecules. The strong streptavidin-biotin bond can highly suited for attaching various biomolecules to one another or onto a solid support. The streptavidin-biotin complex displays high resistance to organic solvents, denaturants, detergents, and proteolytic enzymes. It can also withstand extremes of temperature and pH. However, one challenge faced by researchers using the streptavidin-biotin system is the target aggregation, which results from multivalent binding. It can cause cross-linking that can interfere with observations at the molecular level. These challenges can be overcome by use of monomeric streptavidin (mSA) that displays structural and binding properties that are suitable for multiple applications based on its small size and monovalent biotin binding. The monomeric form allows for association with biotinylated ligands without inducing aggregation that are observed with multivalent interactions. Monomeric form can also be genetically fused to another protein to form a biotin-binding tag. SAvPhire Monomeric Streptavidin is a is a high-affinity recombinant variant of streptavidin that minimizes the risk of aggregation, precipitation, and crosslinking in pre-incubation steps with biotinylated molecules and offers easier access to the desired targets. Unlike other non-engineered monomeric forms, SAvPhire Monomeric Streptavidin displays highly desirable dissociation kinetics (koff =63 x 10-3 min-1 for non-engineered monomeric form and 2.1 x 10-3 min-1 for SAvPhire Monomeric Streptavidin) and longer t1/2 (11 min vs 329 min). It is suitable for multiple immunodetection applications. (Ref.: Kroestch, A., et al., (2018). Appl. Microbiol. Biotechnol., 102(23); 10079-10089).
      References
      Product Information
      FormatHRP
      PresentationMonomeric Streptavidin in buffer containing 0.15 M PBS, 0.5% BSA, 0.4% Bronidox, pH 7.2 without azide with 10% glycerol.
      Applications
      ApplicationSAvPhire Monomeric Streptavidin,Cat.#SAE0108, is a high-affinity recombinant variant of SA that minimizes the risk of aggregation, precipitation, and crosslinking in pre-incubation with biotinylated molecules and offers easier access to the desired targets, tested for use in IHC (Paraffin) & WB.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry (Paraffin) Analysis:

      A 1:500 dilution of Anti-NeuN (ABN78) was used to stain mouse cerebral cortex tissue sections and signal was detected with Goat Anti-Rabbit IgG and SAvPhire Monomeric Streptavidin-Horseradish Peroxidase conjugate (1:100 dilution).


      Evaluated by Western Blotting with Anti-GFAP-Biotin Conjugate (Cat. No. MAB3402B) and visualized with SAVphire-HRP.

      Western Blotting Analysis: Mouse brain tissue lysate was probed with 1:1000 dilution of Anti-GFAP-Biotin Conjugate (Cat. No. MAB3402B) and visualized with SAvPhire Monomeric Streptavidin-HRP conjugate and chemiluminescence detection system.
      Biological Information
      ImmunogenN/A
      ConcentrationPlease refer to lot specific datasheet.
      HostE. coli
      SpecificitySAvPhire Monomeric Streptavidin expressed in E. coli and conjugated with Horseradish peroxidase is suitable for immunodetection applications. SAvPhire Monomeric Streptavidin is expressed with an N-terminal His-tag and a C-terminal FLAG® tag.
      Species Reactivity
      • All
      Species Reactivity NotePredicted to react with all species.
      Antibody TypeMonoclonal Antibody
      UniProt Number
      Molecular Weight15.5 kDa for Savphire monomeric streptavidin (with an N-terminal His-tag and a C-terminal FLAG® tag).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with Anti-GFAP-Biotin Conjugate (Cat. No. MAB3402B) and visualized with SAVphire-HRP.

      Western Blotting Analysis: Mouse brain tissue lysate was probed with 1:1000 dilution of Anti-GFAP-Biotin Conjugate (Cat. No. MAB3402B) and visualized with SAvPhire Monomeric Streptavidin-HRP conjugate and chemiluminescence detection system.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      SAE0108-100UG 04061841276760

      Documentation

      SAvPhire Monomeric Streptavidin peroxidase conjugate Certificats d'analyse

      TitreNuméro de lot
      SAvPhire Monomeric Streptavidin peroxidase conjugate - 3689924 3689924
      SAvPhire Monomeric Streptavidin peroxidase conjugate - 4169998 4169998
      SAvPhire Monomeric Streptavidin peroxidase conjugate - 4203331 4203331
      SAvPhire Monomeric Streptavidin peroxidase conjugate - 4208462 4208462
      SAvPhire Monomeric Streptavidin peroxidase conjugate - 4210246 4210246
      SAvPhire Monomeric Streptavidin peroxidase conjugate - 4301964 4301964
      SAvPhire Monomeric Streptavidin peroxidase conjugate - Q3323378 Q3323378