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662065 Ubiquitin, Methylated, Human, Recombinant

Ubiquitin, Methylated, Human, Recombinant : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Me-Ub

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662065
  
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      Aperçu

      Replacement Information
      Description
      OverviewRecombinant, human methylated ubiquitin. A derivative of ubiquitin in which lysine residues are reductively methylated. Methylated ubiquitin can ligate to protein substrates, but does not form polyubiquitin chains. Protein breakdown rates obtained with methylated ubiquitin are slower than those obtained with unmethylated ubiquitin. Useful for reducing the polyubiquitin chain length and for determining rates of ubiquitin conjugation.
      Catalogue Number662065
      Brand Family Calbiochem®
      SynonymsMe-Ub
      References
      ReferencesHershko, A. and Heller, H. 1991. J. Biol. Chem. 266, 16376.
      Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun. 128, 1079.
      Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz. 91, 570.
      Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem. 106, 186.
      Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem. 254, 4359.
      Product Information
      FormLyophilized solid
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      662065 0

      Documentation

      Ubiquitin, Methylated, Human, Recombinant FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Ubiquitin, Methylated, Human, Recombinant Certificats d'analyse

      TitreNuméro de lot
      662065

      Références bibliographiques

      Aperçu de la référence bibliographique
      Hershko, A. and Heller, H. 1991. J. Biol. Chem. 266, 16376.
      Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun. 128, 1079.
      Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz. 91, 570.
      Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem. 106, 186.
      Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem. 254, 4359.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-September-2008 RFH
      SynonymsMe-Ub
      DescriptionRecombinant, human methylated ubiquitin. A derivative of ubiquitin in which lysine residues are reductively methylated. Methylated ubiquitin can ligate to protein substrates, but does not form polyubiquitin chains. Protein breakdown rates obtained with methylated ubiquitin are slower than those obtained with unmethylated ubiquitin. Useful for reducing the polyubiquitin chain length and for determining rates of ubiquitin conjugation.
      FormLyophilized solid
      Recommended reaction conditionsTypical concentrations for non-rate-limiting support of in vitro conjugation reactions range from 200 µM to 1 mM depending on the reaction conditions.
      Purity≥95% by SDS-PAGE
      SolubilityAqueous buffers (50 mg/ml)
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesHershko, A. and Heller, H. 1991. J. Biol. Chem. 266, 16376.
      Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun. 128, 1079.
      Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz. 91, 570.
      Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem. 106, 186.
      Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem. 254, 4359.