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71892 iFOLD® Protein Refolding System 3

71892
  
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      Replacement Information
      Description
      Overview

      This product has been discontinued.





      The iFOLD® Protein Refolding System 3 is designed to screen for optimal refolding conditions for proteins expressed as inclusion bodies. The iFOLD System 3 utilizes FoldACE™ Reagents, which are novel, detergent-free refolding additives that have been demonstrated to significantly enhance refolding of certain proteins. The kit provides a systematic evaluation of 96 conditions representing different buffering systems, pH values, redox agents, and 5 different FoldACE Reagents. Following cell lysis with the aid of Lysonase™ Bioprocessing Reagent, membrane components and contaminating proteins trapped within inclusion body pellets are removed by a series of washes with Triton X-100. The purified inclusion bodies are reduced and denatured by addition of Tris(2-carboxyethyl) phosphine (TCEP) and guanidine hydrochloride and refolded by rapid dilution into the iFOLD System refolding buffers. Components for cell lysis, inclusion body washes and denaturation, and a pre-dispensed 96-well plate-based matrix of refolding buffers are included in the kit. Each kit is sufficient for screening up to 96 refolding conditions (91 experimental and 5 buffer-only control wells) for a single protein.
      Catalogue Number71892
      Brand Family Novagen®
      References
      Product Information
      DeclarationPatent pending.
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 34

      Causes burns.
      S PhraseS: 26-27-36/37/39

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Take off immediately all contaminated clothing.
      Wear suitable protective clothing, gloves and eye/face protection.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Multiple Storage Conditions
      Toxicity Corrosive
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage Multiple storage requirements
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      71892 0

      Documentation

      iFOLD® Protein Refolding System 3 Certificats d'analyse

      TitreNuméro de lot
      71892

      Protocoles Utilisateur

      Titre
      TB505 iFOLD™ Refolding System 3
      TB509