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71243 pIEx™-3 DNA

pIEx™-3 DNA : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: InsectDirect™ System- pIEx™-3 DNA

71243
  
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      Replacement Information
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      This product has been discontinued.





      The pIEx™ vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1. pIEx-3 contains the coding sequence for the signal peptide of adipokinetic hormone (AKH) to allow secretion of the expressed protein. pIEx-3 also contains N-terminal GST•Tag, His•Tag, and S•Tag coding sequences, and a C-terminal HSV•Tag coding sequence. Unique sites are shown on the circle map. N-terminal vector encoded sequences can be completely removed by cloning into the PshA I site and cleaving the fusion protein with enterokinase.
      pIEx-3 is also available as an Ek/LIC vector. The Ek/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.

      For sequencing or PCR of recombinants in pIEx-3, refer to the table below to choose the appropriate primers.
      Vector5' Primer3' Primeralt 5' Primer
      pIEx-1S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
      pIEx-2S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
      pIEx-3S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
      pIEx-4IE1 Promoter PrimerAS S•Tag 18mer Primer
      NA
      pIEx-5IE1 Promoter PrimerAS S•Tag 18mer Primer
      NA
      pBiEx-1S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
      T7 Promoter Primer
      pBiEx-2S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
      T7 Promoter Primer
      pBiEx-3IE1 Promoter PrimerAS S•Tag 18mer PrimerT7 Promoter Primer
      Catalogue Number71243
      Brand Family Novagen®
      SynonymsInsectDirect™ System- pIEx™-3 DNA
      References
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      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
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      Global Trade Item Number
      Référence GTIN
      71243 0